Dow University Hospital established a huge operation purpose built theater i.e. OT Complex of International standards having 80,000 sq. ft. area which is fully equipped with most modern equipment. The OT Complex has nine 09 operation theatres. Two are designated for Liver Transplant and hepato pancreatic biliary surgeries while two theatres are designated for Cardiac and Orthopedic Surgeries; the required special instruments and gadgets for the purpose are installed and provided.

The remaining five Theatres are also fully equipped with most modern equipment to have operations of General Surgery, Urology, ENT, Plastic Surgery, Neuro Surgery and Thoracic Surgery, Bariatric Surgery etc. This complex also has fully equipped recovery room. The operation floor has a unique channelizing operating in automation to collect the dirty liner with safety, which is then washed, sterilized and stored hygienically preventing infection. 

The OT complex has state of art equipment including ventilators, invasive hemodynamic monitoring system and hemodialysis. Since various specialties also provide Postgraduate training, camera recording and CCTV system is also installed for telecasting live operative procedures. This new OT complex has lot of facilities for the doctors, staff and students, including rest rooms, canteens and separate lockers besides other facilities.