Research Interest

• My Research activities are mainly focused on snake venom proteomics, its toxicities and therapeutic applications. I am also working on using alternate venom neutralizing approaches such as traditional medicines and nanoparticles. Additionally, venom toxicity and its neutralizing agents are assessed using Drosophila melanogaster models. Other research interests include production of industrially important products locally for overall environmental and socio-economic impact for the country.

Research Publications

• • Kainat R, Ahmed I, Alolaywi AM, Waheed H, Sultan ZK, Moin SF. 2023. Assessment of Salivary MMP-8 and IL-1β for the Diagnosis of Periodontal Diseases in Pakistani Population. European Journal of Dentistry. DOI https://doi.org/10.1055/s-0043-1772779.
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• • Khan SK, Waheed H, Moin SF. 2023. Decomplexation of Venom Proteome of Pakistani Cobra (Naja Naja Naja). PPASB. 60, 125-131.
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• • Fazil MM, Javed I, Ali K, Waheed H*, Dastagir N. 2023. Production optimization and industrial applications of amylase from indigenous bacterial species using banana peels. Biosight. 4(1):41-51.
   
• • Khan ZM, Waheed H, Khurshid Z, Moin SF. 2023. The phenotypic polymorphism of saliva from dental caries patients. Biosight. 4(1):33-40.
   
• • Hussain M, Amanullah A, Aslam A, Raza F, Arzoo S, Qureshi IW, Waheed H, Jabeen N, Shabbir S, Sayeed MA, Quraishy S. 2022. Design and immunoinformatic assessment of candidate multivariant mRNA vaccine construct against immune escape variants of SARS-CoV-2. Polymers. 14:3263. 
   
• • Shabbir A, Waheed H*, Ahmed S, Shaikh SS, Ahmed W. 2022. Association of salivary Cathepsin B in different histological grades among patients presenting with oral squamous cell carcinoma. BMC Oral Health. 22:63. 
   
• • Khan ZM, Waheed H, Khurshid Z, Zafar MS, Moin SF, Alam MK. 2021. Differentially expressed salivary proteins in dental caries patients. BioMed Research International. 2021.
   
• • Nazeer M, Waheed H, Saeed M, Ali SY, Choudhary MI, Qasmi ZH Ahmed A. 2019. Purification and characterization of a nonspecific lipid transfer protein 1 (nsLTP1) from Ajwain (Trachyspermum ammi) seeds. Scientific Reports. 9: 4148.
   
• • Waheed H, Doman SK, Ahmed A. 2018. The partial amino acid sequence of Leaf-nosed Viper (Eristicophis macmahonii) snake hemoglobin. The Journal of Animal and Plant Sciences. 28(6):1622-1628.
   
• • Waheed H*, Moin SF, Choudhary MI. 2017. Snake venom: From deadly toxins to life-saving therapeutics. Current Medicinal Chemistry. 24:1-18. 
   
• • Waheed H, Friedman H, Moin SF, Zarina S, Ahmed A. 2016. The primary structure of βI-chain of hemoglobin from snake Sindhi Krait (Bungarus sindanus sindanus). Protein J. 35(3):193-201. 
   
• • Moin SF, Rainer M, Waheed H, Stasyk T, Huber LA, Lottspeich F, Bonn GK. 2011. Purification and characterization of phospholipase A2 and identification of kappa Bungarotoxin from Bungarus sindanus sindanus (Sindhi Krait) venom. Current Analytical Chemistry. 7(3):176-183.

   

Others

Oral Presentation

• • Antidotes from Traditional Medicine: Studies on Snake Venom Neutralization by Datura Alba. 4th International Conference on Emerging Trends in Bioinformatics & Biosciences (ICETBB-2022). Hazara University, Manshera, 15-17th September 2022. 

Participation Outside Country

• • Nazeer M, Waheed H, Lalani R, Choudhary MI, Ahmed A. 2017. Characterization and Molecular Modeling of nonspecific Lipid Transfer Protein 1 (nsLTP1) from Ajwain (Trachyspermum ammi) Seeds. ABRF Annual Meeting. 25th March 2017. San Diego, USA.

• • Doman S, Lalani R, Waheed H, Nazeer M, Choudhary MI, Ahmed A. 2016. Isolation and Purification of Acid Soluble Proteins from Turmeric Root (Curcuma longa) by Multidimensional Chromatography. ABRF Annual Meeting. 20-23rd February 2016. Florida, USA.
   
• • Doman S, Waheed H, Choudhary MI, Ahmed A. 2012. Purification and Partial Characterization of Snake Leaf-Nosed Viper (Eristicophis macmahonii) Hemoglobin. RI-INBRE SURP Meeting. 30th July 2012. Rhode Island, USA.
   
• • Friedman H, Waheed H, Choudhary MI, Ahmed A. 2011. Amino Acid Sequence of Snake Hemoglobin βII Chain from Sindhi Krait (Bungarus sindanus sindanus). 6th BioNES Meeting. 2nd December 2011. Rhode Island, USA.
   
• • Moin SF, Rainer M, Waheed H, Stasyk T, Huber LA, Bonn GK.  2009. Identification of the Phospholipase A2 Component from Snake Bungarus sindanus sindanus Venom Using MALDI-TOF Mass Spectrometry. Euroanalysis. 6-10th September 2009. Innsbruck, Austria.

Grants

• • VCSFI Grant (2022-2023) on “Development of Drosophila melanogaster-based Assay System for Screening Carcinogenic Compounds”.


• • VCSFI Grant (2022-2023) on “Isolation, Purification and Characterization of Bioactive and Anti-cancerous Small Molecules from Oxalis corniculate”.
   
• • HEC Grant (2019-2020) on “Design of Experiment based optimization for lactic acid production using sugar cane molasses as a raw material” Project # 2398.
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• • VCSFI Grant (2023-2024) on “Establishment of Cost-effective Platform for Screening Toxicity of Snake Venom using Drosophila melanogaster”.

 Teaching

• I teach undergraduate courses of Research Methodology and Skill Enhancement, Analytical Chemistry and Instrumentation, Molecular Diagnostics and postgraduate courses of Advances in Biotechnology, Health Biotechnology.

BS Thesis Supervised

• • Titanium Dioxide Nanoparticles as Venom Neutralizing Agents (2023).
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• • Datura alba Seed Extracts and Snake Venom Neutralization (2023).
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• • Silver Nanoparticles as Snake Venom Neutralizing Agents (2023).
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• • Phytochemical Profiling and In-vitro Evaluation of Anti-snake Venom Activity of Ipomea pes caprae (Linn.) (2023).
   
• • Potential of Using Anolis carolinensis as animal model for SARS-CoV2 Pathogenesis (2020).
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• • Potential of Using Xenopus tropicalis as animal model for SARS-CoV2 Pathogenesis (2020).
   
• • Extraction and Evaluation of D. alba Plant Proteins for Various Biological Activities (2021).
   
• • Studies on Biological Activities of Phytochemicals from Datura alba Seeds (2021).
   
• • Partial Purification and Characterization of Bioactive Proteins from Datura alba Seeds (2022).
   
• • Drosophila melanogaster as a Model for Toxicological Profiling of Big Four Venomous Snakes of Pakistan (2022).
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• • Studies on Biological Activities of Phytochemicals from Datura alba Seeds (2021).

MSc Theses Supervised

• •    MPhil (Biotechnology) “Development of Mutagen/Carcinogen Detection System in Drosophila melanogaster” (2023).
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• •    MDS (Oral Surgery) “Investigation of Salivary C-Terminal Telopeptides of Collagen Type I (CTX) Expression Levels with Early and Delayed Loading of Dental Implants Patients” (2023).
   
• • M.Sc. Ds. (Oral Medicine) “Association of Expression of Salivary Leukocyte Protease Inhibitor (SLPI) in Metastasis of Oral Cancer” (2020).
   
• • M.Sc. Ds. (Oral Medicine) “Comparison of Salivary Cathepsin B Levels in Oral Squamous Cell Carcinoma” (2021). 

Honors & achievements

• • Azhar Z, Siddiqui I, Khan N, Waheed H*, Faraz Moin. 2022. “Biosafe Stain for Polyacrylamide Gels: An Environmentally Friendly Approach”. In: 7th All Pakistan DUHS–DICE Health Innovation Exhibition, Karachi 4th November 2022. (Special Prize).
   
• • Tahir W, Fatima SM, Moin M, Dastagir N, Waheed H*. 2022. "Study on Biological Activities of Phytochemicals and Proteins from Datura alba Seeds”. In: 1st International Conference of Biotechnology, University of Karachi 16-17th March 2022. (3rd Prize).
   
• • Rehman A, Baig AA, Gulzar M, Sarwar L, Waheed H*, Ahmed I. 2018. “Production of Protease Enzyme Through Different Bacterial and Fungal Isolates by Using Industrial Wastes”. In: International Conference on Innovation and Commercialization in Biotechnology, PCSIR, Lahore 25-26th April 2018. (5th Prize).
   
• • Visiting Research Scholar, Department of Biomedical & Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, USA (Nov 2011-Jan 2012).
   
• • Visiting Research Scholar, European Molecular Biology Laboratories (EMBL), Grenoble, France (Oct 2009-Jun 2010).
   
• • Waheed H, Moin SF, Abbasi A, Ali SA. 2007. “A Molecule to Breathe with: Characterization of Hemoglobin from Leaf-Nosed Viper (E. macmahonii) and Sindh Krait (B. sindanus sindanus)”. In: 9th International Symposium on Protein Structure-Function Relationship, ICCBS 11-14th January 2007. (1st Prize).

Professional Learned Society

• • Member, Scientific Committee, DRIBBS-DUHS.

• • Life Time Member of the Pakistan Biological Safety Association (PBSA).
   
• • Life Time Member of the Chemical Society of Pakistan (CSP).
   
• • Member Pak-France Alumni Network
   
• • Member, Board of Studies, DCoB-DUHS
• • HEC Approved PhD Supervisor in the discipline of Biological Sciences.