Research Interest

• My area of interest is Health Biotechnology with more inclination towards Advanced Cell and Molecular Biology, Genetic Engineering and Clinical Biochemistry. My research involves the significance of Cellular senescence, Genome integrity and the prominent role of DNA damage response in response to chemical and biological mutagens.

Research Publications

• •Fatima T, Amin R, Nadeem J, Khalid A, Khan MM, Shaikh A. Analysis of GATA-1, FoxO3 and pRb Gene expression and assessment of longevity associated FoxO3 rs2802292 polymorphism in transfusion dependent β-thalassemia major patients. Journal of Xi’an Shiyou University, Natural Science Edition. VOLUME 20 ISSUE 03 MARCH 2024.
   
• •Neha Baqai, Rafat Amin, Tehseen Fatima, Zeba Ahmed, Nousheen Faiz. Expression Profiling of EMT Transcriptional Regulators ZEB1 and ZEB2 in Different Histopathological Grades of Oral Squamous Cell Carcinoma Patients. Current Genomics. 15 February 2024.
   
• •T Fatima, S Khan, MM Khan, R Kamran, MW Uddin, S Sohrab. Oxidative Stress in Beta-thalassemia Patients: Role of Enzymatic and Non-enzymatic Modulators. Protein and Peptide Letters 30 (12), 1030-1037.
   
• •Kulsoom Bibi, Tehseen Fatima, Saba Sohrab, Ghulam Haider, Shamshad Zarina, Amber Ilyas. Polymorphic Variants of ASS1 Gene Related to Arginine Metabolism and the Risk of HCC. Protein and Peptide letters. May 23

• •Fatima, T., Khan, S., Khan, M. M., Kamran, R., Uddin, M. W., & Sohrab, S. (2023). Oxidative Stress in Beta-thalassemia Patients: Role of Enzymatic and Non-enzymatic Modulators. Protein and Peptide Letters, 30(12), 1030-1037.
   
• • Sahar N, Arif S, Iqbal S, Riaz S, Fatima T, Ara J, Banks J. Effect of drying surfaces & physical attributes on the development of Aflatoxins (AFs) in red chilies. Journal of Food Processing and Preservation.:e16173.
   
• • Majeed MM, Ahmed I, Roome T, Fatima T, Amin R. Association between Interleukin-1β Gene Polymorphism and Chronic Periodontitis. European Journal of Dentistry. 2021 Jul 24.
   
• • Amin R, Khan S, Zeb TF, Ali S, Baqai N, Baqai M, Shuja S. Knowledge and attitudes toward genetically modified (GM) food among health sciences university students in Karachi, Pakistan. Nutrition & Food Science. 2021 May 5.
   
• • Zehravi M, Wahid M, Ashraf J, Fatima T. Whole-Exome Sequencing Identifies Small Mutations in Pakistani Muscular Dystrophy Patients. Genetic Testing and Molecular Biomarkers. 2021 Mar 1;25(3):218-26.
   
• • Mehmood A, Zeb TF*, Amin R, Waseem HF, Anjum F, Mahmood A, Shaikh A. Effects of Carbonated Drinks on Bone Health of Young Adults: a study conducted amongst students of Health University. Pakistan Journal of Medical & Health Science. 2020. 14(4):850
   
• • Sadaf Khan, Basma Aziz, Urooj Ishrat, Tehseen Fatima Zeb, Rafat Amin, Shaukat Ali. Comparative Evaluation of Salivary Protein Profile and Oxidative Stress Status among Smokers/ Non-Smokers. Pakistan Journal of Medical & Health Science. 2020. 14(4):755 4
   
• • Khan S, Amin R, Ali S, Zeb TF, Haider MS, Tarar OM. Comparative analysis of the nutritional components of different parts of Moringa oleifera from Karachi, Pakistan. Pakistan Journal of Science. 2020 Dec 31;72(4):271.
   
• • Ali S, Amin R, Zeb TF*, Khalid X, Ashar R, Uddin SM, Ali A, Haider MS. Formulation of a cost-effective medium to support high biomass production and improved amino acid profile of Arthrospira platensis. Pakistan Journal of Science. 2020 Dec 1;72(4).
   
• • Abid, A., Khan, H., Fatima Zeb, T*., Uddin, S. M., Amin, R., Khan, S., & Ali, S. (2020). Macromolecular characterization and growth kinetics of Arthrospira platensis and Chlorella Vulgaris as affected by different media (synthetic and Anaerobic Digestor Effluent) - A comparative study. International Journal of Endorsing Health Science Research (IJEHSR), 8(4), 188-201.
   
• • Zaheer, S., Fatima Zeb, T*., Baloch, A., Amin, R., Amjad, Z., & Sheikh, A. (2020). The multifactorial burden of Type 2 Diabetes mellitus: a study from clinical settings of University Hospital, Karachi, Pakistan. International Journal of Endorsing Health Science Research (IJEHSR), 8(4), 275-287.
   
• • Amjad, Z., Shabbir, S., Mughal, A., Zeb, T., Amin, R., & Mirza, T. (2020). Effect of Ginkgo Biloba Extract (EGb 761) on Lead-induced Nephrotoxicity in Wistar Albino Rats. International Journal of Endorsing Health Science Research (IJEHSR), 8(4), 202-211.
   
• • Arzoo S, Naqvi Z, Hussain M, Shamim S, Zeb TF, Ali S. Production and antimicrobial activity of silver nanoparticles synthesized from indigenously isolated Pseudomonas aeruginosa from Rhizosphere. Pak. J. Pharm. Sci. 2020 Nov;33(6):2815-22.
   
• • Muhammad SD, Khan H, Hussain M, Zeb TF, Kumar D, Rahi R, Asif M, Balooch AA. Pharmacogenetics of sulfonylurea: Presence of CYP2C9* 2, CYP2C9* 3 and a novel allele, CYP2C9* 61, in Type 2 diabetes patients under sulfonylurea therapy. Pakistan journal of pharmaceutical sciences. 2020 Jul 1;33(4):1771-7.
   
• • Grazielle-Silva V, Zeb TF, Burchmore R, Machado CR, McCulloch R, Teixeira SM. Trypanosoma brucei and Trypanosoma cruzi DNA mismatch repair proteins act differently in the response to DNA damage caused by oxidative stress. Frontiers in cellular and infection microbiology. 2020 Apr 16; 10:154.
   
• • AbbuHazim F, Ishrat U, Fatima T, Baqai N, Amin R. Evaluating the status of vitamin 25 (OH) D levels among females of all age groups in Karachi, Pakistan. Journal of Muhammad Medical College, Mirpurkhas. 2019; 10(2):55-8.
   
• • Roome T, Aziz S, Razzak A, Aslam Z, Jamali KS, Sikandar B, Fatima T, Abidi L, Imran M, Faizi S, Shah MR. Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4. Biomedicine & Pharmacotherapy. 2019 Apr 1; 112:108624.
   
• • Grazielle-Silva V, Zeb TF, Bolderson J, Campos PC, Miranda JB, Alves CL, Machado CR, McCulloch R, Teixeira SM. Distinct phenotypes caused by mutation of MSH2 in trypanosome insect and mammalian life cycle forms are associated with parasite adaptation to oxidative stress. PLoS neglected tropical diseases. 2015 Jun 17; 9(6):e0003870.

Others

Oral Presentation

• Exploring the molecular mechanism involved in sudden weight loss and alveolar damage in pulmonary tuberculosis patients. BIOCON-2, 5-6th March 2024, University of Karachi, Karachi.

• Mismatch Repair (MMR) in Androgen Receptor: Expression of EMT Transcriptional Regulators (ZEBs’) in Relation to the Tri-nucleotide Repeats in OSCC Patients. 11th International conference on biological and computational sciences. (C-BICS 2023). October 28, 2023.

• Exploring the molecular mechanism involve in sudden weight loss and alveolar damage in Pulmonary Tuberculosis patients. 3rd Annual Biological Sciences Conference, AKUH, 15th Nov. 2023, Ayesha Aafsar, Tehseen Fatima

• • Cardio-renal syndrome and inflammatory oxidative stress: A crosstalk in beta-thalassemia patients. 1st conference of lifscicon, SZABIST, Karachi, 10th – 11th may, 2022, Muhammad Mubashir Khan, Tehseen Fatima.
   
• • Expression Analysis of AKT/mTOR signaling pathway: an evaluation of CRS risk factors in beta-thalassemia patients. 4th international conference on emerging trends in Bioinformatics & Biosciences, Hazara University, Mansehra, 18th October, 2022, Muhammad Mubashir Khan, Tehseen Fatima.
   
• • Cellular Senescence and Genomic Instability: The Role of Oxidative Stress in β-Thalassemia Major Patients. 4th international conference of Bio-informatics & Bio-sciences, Hazara University, Mansehra, 18th October, 2022, Tehseen Fatima.
   
• • Rationalizing the potential role of erythropoiesis regulatory genes pRB, GATA-1 and FOXO3 in β-thalassemia major patients. 1st conference of lifscicon, SZABIST, Karachi, 10th – 11th May, 2022, Muhammad Wajih Uddin, Dr Tehseen Fatima.
   
• • Mismatch repair (MMR) in androgen receptor: expression of EMT transcriptional regulators (ZEB’S) in relation to the tri-nucleaotide repeats in OSCC patients. 4th international conference on emerging trends in Bioinformatics & Biosciences, Hazara University, Mansehra, 18th October, 2022, Neha Baqai, Tehseen Fatima.

Poster Presentations

• • Characterization and Partial purification of Bacteriocin Produced by Lactococcus gravieae for Food preservation. Dow Annual Research Day, 30th November, 2023 Amna Naz, Tehseen Fatima.
   

• • Exploring the molecular mechanism involve in sudden weight loss and alveolar damage in Pulmonary Tuberculosis patients. Dow Annual Research Day, 30th November, 2023, Ayesha Aafsar, Tasbie Zehra, Tehseen Fatima
   

• • Map Kinase Dysregulation-induced Cell Cycle Arrest In COVID-19 Infection. Dow Annual Research Day, 30th November, 2023, Hira Abbas, Tehseen Fatima.
   

• • Characterization and Partial purification of Bacteriocin Produced by Lactococcus gravieae for Food Preservation, 3rd Annual Biological Sciences Conference, AKUH, 15th Nov. 2023. Amna Naz, Tehseen Fatima.

• • Map Kinase Dysregulation-induced Cell Cycle Arrest In COVID-19 Infection, 3rd Annual Biological Sciences Conference, AKUH, 15th Nov. 2023. Hira Abbas, Tehseen Fatima.
   

• • Partial Purification and characterization of Bacteriocin from Lactobacilli isolated from food samples. 4th international conference on emerging trends in Bioinformatics & Biosciences, Hazara University, Mansehra, 18th October, 2022, Amna Naz, Tehseen Fatima.
   
• • Genomic Integrity At Risk In Β- Thalassemia Patients– A Coalesce Of Markers Of Oxidative Stress And Metabolic Desrgulation, 1st international conference of biotechnology, BIOCON-1 2022, university of Karachi, Karachi, Tayyaba Hashmi, Tehseen Fatima.
   
• • Mismatch repair in T. brucei: Roles in protection against oxidative damage. KMCBM, Marine Biological Laboratory, Woods Hole, MA, USA, from April 21-25th 2013. Tehseen Fatima, Richard McCulloch

Grants

• •NIH (SG-22/R3-27, March 2023)
   
• •NIH (SG/22/R3-20/RDC/1524, March 2023)
   
• •Research Grant from Health Research Institute (HRI), National Institute of Health (NIH), Islamabad-(2023)
   
• •VCSFI Research Grant (2019B-0072)

Teaching

• My teaching responsibilities include classes and lab demonstrations of multiple courses offered at Dow College of Biotechnology for undergraduate program. These courses include Cell Biology, Molecular Biology, Methods in Molecular Biology, Biochemistry I, and Biochemistry II. Furthermore I am also involved in teaching of postgraduate courses including Medical genetics, Advances in Biotechnology, Health Biotechnology, Fundamentals of Biotechnology and Fundamentals of Molecular Biology.
   

BS Research Project/ Thesis Supervisor

• • Characterization of salivary protein profile and oxidative stress status among young healthy smokers and non-smokers.(2019)
   
• • Rationale of oxidative stress and associated DNA repair mechanisms in β thalassemia patients.(2020)
   
• • Metabolic Dysregulation in β-Thalassemia Major: A cross talk between metabolic markers and cell cycle.(2020)
   
• • ROS production in Beta thalassemia patients: The impact of Vitamin D receptor polymorphism and HuR dependent SOD2 expression.(2021)
   
• • Rationalizing the potential role of erythropoiesis regulatory genes pRB, GATA-1 and FOXO3 in β-thalassemia major patients.(2021)
   
• • Evaluation of oxidative stress status by expression analysis of NOS-2, HuR and SOD-2 in COVID-19 patients.(2022)
   
• • Expression analysis of apoptotic signaling pathway biomarkers (P53, AKT, MAPK) in patients at various stages of SARS-CoV2 infection.(2022)
   
• • Role of ACE2, Nrf2, GPx and Angiotensin II in progression of COVID-19: Insights on Inflammation induced oxidative stress in COVID-19 Patients.(2022)
   
• • Expression Analysis of cell cycle regulators in various stages of SARS-cov2 infection.
   
• • Novel outlook on SARS CoV-2 disease progression in the light of CDKs.(2022)
   
• • Cell cycle arrest and COVID-19: AN expression analysis of pRB & E2F levels in various stages of SARS-CoV2 infection.(2022)
   
• • Bacteriocin Production by Lactococcus gravieae: Characterization, Partial Purification, and Probiotic Assessment of Indigenous Isolates from Gallus gallus domesticus. (2023)
   
• • Exploring The Molecular Mechanism Involve In Sudden Weight Loss In Pulmonary Tuberculosis Patients (2023)
   
• • Exploring the molecular mechanism involved in alveolar damage in Pulmonary Tuberculosis (2023)
   
• • Synthesis of Silica Nanoparticles from onion peel and sugarcane bagasse (2023)

M.S. Thesis Supervisor

• • MSc. (Biotehnology) Biochemical Analysis and characterization of C. vulgaris grown in synthetic media and anaerobic digester effluent: a comparative study.(2019)
   
• • MSc. (Biotechnology) Biochemical Profile and growth kinetics of A. platensis as affected by different media (ADE and ZK): A comparative study. (2019)
   
• • MS. (BioSciences) Identification of rare SNV in GLP1R in association of type 2 Diabetes susceptibility in a sub group of Pakistani population. 2020

M.Phil. Thesis Supervisor

• •M.Phil. (Biotechnology) “Evaluation of Iron, Hepcidin and IL-10 in Relation to Body Mass Index in Young Healthy Premenopausal Women” (2023).
   
• •M.Phil. (Biotechnology) “Expression analysis of AKT/mTOR signaling pathway: An evaluation of cardiorenal syndrome risk factors in Beta-thalassemia major patients”
   
• • MPhil (Biotechnology) The impact of Vitamin D receptor (VDR) gene polymorphisms, VDR expression and Vitamin D status on modifiable atherosclerotic metabolic risk factors in young healthy Pakistani adults. 2022
   
• • MPhil (Biotechnology) Mismatch Repair (MMR) in Androgen Receptor; Expression of EMT Transcriptional Regulators (ZEBs’) in Relation to the Tri-nucleotide Repeats in OSCC Patients.
   
• • MPhil (Genetics) Identification of DNA polymorphisms at specific site loci in β-thalassemia and compound heterozygote of Sickle/β thalassemia patients.2022
   
• • MPhil. (Physiology) Investigating the expression patterns of Androgen Receptor in different grades of Oral Squamous Cell carcinoma. 2022

Honors & achievements

• • Merit Certificate for 3rd Position in MSc.(2004) Department of Biotechnology, University of Karachi, Pakistan
   
• • Ph.D. Scholarship (2008) Higher Education Commission of Pakistan, Pakistan

Professional Learned Society

• Membership of British Society of Parasitology (BSP)

Research Fellowship

•  “PhD Research Fellow” at Wellcome Trust Centre of Molecular Parasitology Sir Graeme Davies Building, University of Glasgow (UK)